phage screening

Phage display technology is one of the major approaches employed in various protein interaction studies, especially in the discovery of specific antibodies, scaffolds or ligands. While binders with the desired specificity generally exist at low frequencies in the constructed libraries. In this way, phage display library screening has become an effective technique for the enrichment and identification of binders with high affinity and specificity from interested libraries.

At Creative Biolabs, the proprietary protocol and tailored biopanning strategy allow the increase of candidates with higher affinity and desired specificity (e.g. binders recognize conformational epitope rather than linear epitope). In general, by conducting four rounds library screening, our scientists can select scFv/Fab antibodies with the affinity of 10-7. Through constructing serial sub-libraries of the isolated scFv/Fab antibodies, we can increase the affinity from 10-8 to 10-9. Moreover, Creative Biolabs has successfully obtained a scFv antibody with an extremely high affinity of 10-12.

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