Co IP Protocol
1 RESULTS
45-1 Ramsey Road, Shirley, NY 11967, USA, Shirley
Tel: 6316197922
Co-Immunoprecipitation (Co-IP) is an extension of immunoprecipitation (IP) with which Co-IP shares the same fundamental principle of the specific antigen-antibody reaction. By targeting a known protein with a specific antibody it may be possible to pull the entire protein complex out of solution thereby identifying unknown members of the complex. However Co-IP requires greater care and more physiologically relevant conditions than traditional IP. When successful Co-IP pulls down not only the protein of interest but also its interaction partners. Thus Co-IP is a powerful technique to identify protein complexes and determine protein-protein interactions. Here we provide a detailed procedure for... read more
Tel: 6316197922
Co-Immunoprecipitation (Co-IP) is an extension of immunoprecipitation (IP) with which Co-IP shares the same fundamental principle of the specific antigen-antibody reaction. By targeting a known protein with a specific antibody it may be possible to pull the entire protein complex out of solution thereby identifying unknown members of the complex. However Co-IP requires greater care and more physiologically relevant conditions than traditional IP. When successful Co-IP pulls down not only the protein of interest but also its interaction partners. Thus Co-IP is a powerful technique to identify protein complexes and determine protein-protein interactions. Here we provide a detailed procedure for... read more
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